ATP biotech Inc.

Introduction

Format: 96-Well
Sample: 200μl of serum, plasma, body fluids and cell culture supernatant
Operation: Centrifuge / vacuum manifold
Binding capacity :up to 30µg per well
Recovery Rate > 90% recovery
Operation time : 40 minutes
Application: RT-PCR, PCR, Real-time PCR, Real-time RT-PCR, Automated fluorescent DNA sequencing

ATP™96-Well Viral DNA/RNA Extraction Kit is specially designed for high-throughput purification of viral RNA or DNA from cell-free samples as serum, plasma, body fluids and the supernate of viral infected cell culture. With the extraction method, DNA/RNA viruses are lysed quickly and efficiently by lysis buffer which is a highly concentrated solution of chaotropic salt. Nucleic acid in chaotropic salt and methanol are bond to the glass-fiber matrix of viral DNA/RNA Binding Column. Contamina-tions like salts, metabolites and soluble macromolecular components are removed in Washing Steps. The nucleic acids can be eluted by low-salt buffer or water and are ready-to-use in subsequent reactions. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assay. This protocol is recommended for parallel purification of viral RNA including HCV , HIV and viral DNA including HBV and CMV.

Kit Contents：Cat.No. / Kit Contents

AVR050 (50 preps/kit)	AVR100 (100 preps/kit)
VB Lysis Buffer: 30 ml AD Buffer(concentrated) : 4 ml W1 Buffer : 30 ml Wash Buffer(concentrated) : 12.5 ml RNase-free water:6 ml VB columns : 50 pcs (yellow/white filter) 2ml Collection Tubes : 100 pcs	VB Lysis Buffer: 60 ml AD Buffer(concentrated) : 8 ml W1 Buffer : 50 ml Wash Buffer(concentrated) : 25 ml RNase-free water:6 ml VB columns : 100 pcs (yellow/white filter) 2ml Collection Tubes : 200 pcs