Introduction
Format: Spin columns
Sample: Up to 25 mg of tissue
Operation: Centrifuge / vacuum manifold
Recovry : Up to 25 μg
Application: RT-PCR、Real-time PCR、Notthern blotting、mRNA selection、cDNA synthesis、Primer extension
 


ATP™Total RNA Mini Kit (Tissue) is specially designed for purification of total RNA from a variety of animal tissues or cells.

1.  The provided micropestle can efficiently homogenize tissue samples in the microcentrifuge tube.

2.  The method uses detergent and a chaotropic salt to lyse cells and inactivate RNase.

3.  Then RNA in the chaotropic salt is bonded to the glass fiber matrix of the column.

4.  After washing off the contaminants, the purified RNA is eluted by RNase-free water.

The entire procedure can be completed in 20 minutes and the purified RNA is ready for RT-PCR, Northern blotting, primer extension and cDNA library construction.

 
 
Kit Contents:Cat.No. / Kit Contents
ART50 (50prep/kit )
ART100 (100 prep/kit )
RB Buffer :30ml
W1 Buffer :30ml
Wash Buffer (concentrated)*:12.5ml
RNase-free water:6 ml
RB Columns:50 pcs (yellow/white filter)
Filter Column:50 pcs
2 ml Collection Tubes:100pcs
Micropestle :50pcs
RB Buffer :60ml
W1 Buffer :50ml
Wash Buffer (concentrated)*:25ml
RNase-free water:6 ml
RB Columns:100 pcs (yellow/white filter)
Filter Column:100 pcs
2 ml Collection Tubes:200pcs
Micropestle :100pcs
 
 
 
 
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
 
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
 
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
 
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
 
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
 
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis