ATP™Total RNA Mini Kit (Tissue) is specially designed for purification of total RNA from a variety of animal tissues or cells.
1. The provided micropestle can efficiently homogenize tissue samples in the microcentrifuge tube.
2. The method uses detergent and a chaotropic salt to lyse cells and inactivate RNase.
3. Then RNA in the chaotropic salt is bonded to the glass fiber matrix of the column.
4. After washing off the contaminants, the purified RNA is eluted by RNase-free water.
The entire procedure can be completed in 20 minutes and the purified RNA is ready for RT-PCR, Northern blotting, primer extension and cDNA library construction.