ATP biotech Inc.

Introduction

Format: Spin columns
Sample: Up to 25 mg of tissue
Operation: Centrifuge / vacuum manifold
Recovry : Up to 25 μg
Application: RT-PCR、Real-time PCR、Notthern blotting、mRNA selection、cDNA synthesis、Primer extension

ATP™Total RNA Mini Kit (Tissue) is specially designed for purification of total RNA from a variety of animal tissues or cells.

1. The provided micropestle can efficiently homogenize tissue samples in the microcentrifuge tube.

2. The method uses detergent and a chaotropic salt to lyse cells and inactivate RNase.

3. Then RNA in the chaotropic salt is bonded to the glass fiber matrix of the column.

4. After washing off the contaminants, the purified RNA is eluted by RNase-free water.

The entire procedure can be completed in 20 minutes and the purified RNA is ready for RT-PCR, Northern blotting, primer extension and cDNA library construction.

Kit Contents：Cat.No. / Kit Contents

ART50 (50prep/kit )	ART100 (100 prep/kit )
RB Buffer ：30ml W1 Buffer ：30ml Wash Buffer (concentrated)*：12.5ml RNase-free water：6 ml RB Columns：50 pcs (yellow/white filter) Filter Column：50 pcs 2 ml Collection Tubes：100pcs Micropestle ：50pcs	RB Buffer ：60ml W1 Buffer ：50ml Wash Buffer (concentrated)*：25ml RNase-free water：6 ml RB Columns：100 pcs (yellow/white filter) Filter Column：100 pcs 2 ml Collection Tubes：200pcs Micropestle ：100pcs