Format: Spin columns
Sample: 500 mg plant tissue
Operation: Centrifuge / vacuum manifold
Operation time: < 60 minutes
Yield: 5-300 μg
Elution volume: 50 µl
Application: RT-PCR、Real-time PCR、Notthern blotting、mRNA selection、cDNA synthesis、Primer extension

ATP™ Plant Total RNA Mini Kit provides a fast and simple method to isolate total RNA from plant tissue and cells. In the process, sample is first ground in liquid nitrogen and filtrated by filter column to remove cell debris. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column. The optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer. Finally, the purified total RNA is eluted by RNase-free water. The protocol does not require phenol extraction and alcohol precipitation.

Quality Control

The quality of Plant Total RNA Mini Kit is tested on a lot-to-lot basis. The Kits are tested by isolation of total RNA from 25 mg young leaf. Purified RNA could be quantified with spectrophotometer and checked by agarose gel.

Kit Contents:Cat.No. / Kit Contents
ARPM010 (10 prep/kit )
RB Buffer :60 ml
PRB Buffer :60 ml
W1 Buffer :50 ml
Wash Buffer (concentrated)*:25 ml
RNase-free water:6 ml
RB Columns:10 pcs
Filter Column:10 pcs
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis