ATP biotech Inc.

Introduction

Format ： Ion-exchange resin column
Sample ： 25-300ml bacterial culture
Binding capacity ： Midi column is 500μg / Maxi column is1mg
Operation ： Gravity-flow
Operation time ： 120 minutes
Purity ： Equity to that obtained by 2xCsCl-gradient centrifugation
Application ： Transfection；Microinjection；Sequencing；Restriction Digestion；In vitro Transcription

ATP™ Plasmid Midi/Maxi Kits use pre-packed anion-exchange resin column to purify plasmid or cosmid DNA from bacterial cultures. In the process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. By a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with the Wash Buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in 120 minutes without ultracentrifuges and HPLC or other toxic reagents.

Quality Control

The quality of ATP™ Plasmid Midi/Maxi Kits is tested on a lot-to-lot basis. The Kits are tested by isolation of plasmid DNA from 50ml/100ml culture of E.coli DH5α transformed with the plasmid pBluescript (A600 >2 units/ml). More than 120μg/400μg of plasmid DNA could be quantified with spectrophotometer. 1 µ g of the purified plasmid is used on restriction enzyme digestion with EcoRI and digested DNA is checked by agarose gel analysis.

Kit Contents：Cat.No. / Kit Contents

APDIO25 (25 preps/kit)
PD1 Buffer : 110 ml PD2 Buffer : 110 ml PD3 Buffer : 110 ml PEQ Buffer : 130 ml PW Buffer(concentrated) : 360 ml PEL Buffer : 220 ml RNase A (50mg/ml):200μg Plasmid-Midi Columns: 25 pcs