Format : Ion-exchange resin column
Sample : 25-300ml bacterial culture
Binding capacity : Midi column is 500μg / Maxi column is1mg
Operation : Gravity-flow
Operation time : 120 minutes
Purity : Equity to that obtained by 2xCsCl-gradient centrifugation
Application : Transfection;Microinjection;Sequencing;Restriction Digestion;In vitro Transcription


ATP™ Plasmid Midi/Maxi Kits use pre-packed anion-exchange resin column to purify plasmid or cosmid DNA from bacterial cultures. In the process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. By a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with the Wash Buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in 120 minutes without ultracentrifuges and HPLC or other toxic reagents.


Quality Control

The quality of ATP™ Plasmid Midi/Maxi Kits is tested on a lot-to-lot basis. The Kits are tested by isolation of plasmid DNA from 50ml/100ml culture of E.coli DH5α transformed with the plasmid pBluescript (A600 >2 units/ml). More than 120μg/400μg of plasmid DNA could be quantified with spectrophotometer. 1 µ g of the purified plasmid is used on restriction enzyme digestion with EcoRI and digested DNA is checked by agarose gel analysis.

Kit Contents:Cat.No. / Kit Contents
APDIO25 (25 preps/kit)
PD1 Buffer : 110 ml
PD2 Buffer : 110 ml
PD3 Buffer : 110 ml
PEQ Buffer : 130 ml
PW Buffer(concentrated) : 360 ml
PEL Buffer : 220 ml
RNase A (50mg/ml):200μg
Plasmid-Midi Columns: 25 pcs
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis