ATP biotech Inc.

Introduction

Format: Spin columns
Sample: Blood, other body fluids and cultured cells
Sample size: Up to 300μl / 2ml / 10ml blood
Operation: Centrifuge / vacuum manifold
Operation time: 20 minutes
Binding capacity: 50µg~500µg DNA
Application: PCR、Real-time PCR、Southern blotting、AFLP、PADP/ AFLP

ATP™ Genomic DNA Mini Kit (Blood/Cultured Cell/Bacteria) provide a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood, plasma, serum, buffy coat, other body fluids, lymphocytes, bacterial and cultured cells. In this procedure, RBC Lysis Buffer is used to remove non-nucleated red blood cells and reduce hemoglobin contamination. The method use a chaotropic salt, guanidine hydrochloride to lyse cells and degrade protein, than DNA in chaotropic salt is bond to glass fiber matrix of column. After washing off the contaminants, the purified genomic DNA is eluted by low salt elution buffer or water. The entire procedure can be completed in 40 minutes without phenol/chloroform extraction and alcohol precipitation. Average yield are 6 µ g of DNA from 200 µ l of human whole blood and up to 50 µ g of DNA from 200 µ l of buffy coat, 5 x 10 6 lymphocyte cells, or cultured cells. Purified DNA with approximate 20-30kb is suitable for PCR or other enzyme reaction.

Expectant yield:

Quality Control

The quality of ATP™ Genomic DNA Kit (Blood/Culture Cell/Bacteria) is tested on a lot-to-lot basis. The Kits are tested by isolation of genomic DNA from 200 μ l of human whole blood. The purified DNA is quantified with spectrophotometer and the yield of genomic DNA is 4~6 μg with A260/A280 ratio 1.6 to 1.8.

Kit Contents：Cat.No. / Kit Contents

AGB100 (100preps/ kit )	AGB300 (300preps/ kit )
RBC lysis Buffer ：120ml GT Buffer ：30ml GB Buffer ：30ml W1 Buffer ：45ml Wash Buffer (concentrated)*：25ml Elution Buffer：30ml GD Columns：100pcs (green/white filter) Collection Tubes：200pcs	RBC lysis Buffer ：360ml GT Buffer ：75ml GB Buffer ：75ml W1 Buffer ：130ml Wash Buffer (concentrated)**：50ml Elution Buffer：75ml GD Columns：300pcs (green/white filter) Collection Tubes：600pcs