Format: Spin columns
Sample: Blood, other body fluids and cultured cells
Sample size: Up to 300μl / 2ml / 10ml blood
Operation: Centrifuge / vacuum manifold
Operation time: 20 minutes
Binding capacity: 50µg~500µg DNA
Application: PCR、Real-time PCR、Southern blotting、AFLP、PADP/ AFLP


ATP™ Genomic DNA Mini Kit (Blood/Cultured Cell/Bacteria) provide a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood, plasma, serum, buffy coat, other body fluids, lymphocytes, bacterial and cultured cells. In this procedure, RBC Lysis Buffer is used to remove non-nucleated red blood cells and reduce hemoglobin contamination. The method use a chaotropic salt, guanidine hydrochloride to lyse cells and degrade protein, than DNA in chaotropic salt is bond to glass fiber matrix of column. After washing off the contaminants, the purified genomic DNA is eluted by low salt elution buffer or water. The entire procedure can be completed in 40 minutes without phenol/chloroform extraction and alcohol precipitation. Average yield are 6 µ g of DNA from 200 µ l of human whole blood and up to 50 µ g of DNA from 200 µ l of buffy coat, 5 x 10 6 lymphocyte cells, or cultured cells. Purified DNA with approximate 20-30kb is suitable for PCR or other enzyme reaction.

Expectant yield:

Quality Control

The quality of ATP™ Genomic DNA Kit (Blood/Culture Cell/Bacteria) is tested on a lot-to-lot basis. The Kits are tested by isolation of genomic DNA from 200 μ l of human whole blood. The purified DNA is quantified with spectrophotometer and the yield of genomic DNA is 4~6 μg with A260/A280 ratio 1.6 to 1.8.

Kit Contents:Cat.No. / Kit Contents
AGB100 (100preps/ kit )
AGB300 (300preps/ kit )
RBC lysis Buffer :120ml
GT Buffer :30ml
GB Buffer :30ml
W1 Buffer :45ml
Wash Buffer (concentrated)*:25ml
Elution Buffer:30ml
GD Columns:100pcs (green/white filter)
Collection Tubes:200pcs
RBC lysis Buffer :360ml
GT Buffer :75ml
GB Buffer :75ml
W1 Buffer :130ml
Wash Buffer (concentrated)**:50ml
Elution Buffer:75ml
GD Columns:300pcs (green/white filter)
Collection Tubes:600pcs
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis