ATP biotech Inc.

Introduction

Format: Spin column
Sample: Up to 300 mg agarose gel slice; Up to 100 µl PCR product or other enzymatic reaction
Operation: Centrifuge / vacuum manifold
DNA size: 50 bp ~ 10 kb
Operation time: 20 minutes for gel extraction; 15 minutes for PCR clean up
Expectant recovery: 75-85% for gel extraction; 85-95% for PCR clean up
Application: DNA Sequencing; Ligation; PCR；Restriction Digestion; DNA labeling

ATP™ Gel/PCR DNA Fragments Extraction Kit is designed to recover or concentrate DNA fragments ( 50 bp-10 kb ) from agarose gel, PCR or other enzymatic reaction.

1. The method uses a chaotropic salt,guanidine thiocyanante , to dissolve the agarose gel and denature enzymes.

2. The DNA fragments in the chaotropic salt are then bound to the uniquely designed glass fiber matrix in the spin column in the optimized salt concentration and pH provided by our buffer.

3. Whereas unwanted impurities, such as salts, enzymes, primers unincorporated nucleotides, dyes, and ethidium bromide flow through the column and are easily and efficiently removed from reaction mixture.

4. After washing off contaminants, the purified DNA fragments are eluted by a low salt elution buffer or water.

The entire procedure does not require DNA phenol extraction and alcohol precipitation , and could be completed in 20 minutes .

Quality Control

The quality of ATP™ Gel/PCR fragments Extraction Kit is tested on a lot-to-lot basis. The efficiency of DNA recovery is tested by isolation of DNA fragment of various sizes from either aqueous solution or agarose. The purified DNA is checked by agarose gel analysis.

Kit Contents：Cat.No. / Kit Contents

APF100 (100 preps/kit)	APD300 (300 preps/kit)
DF Buffer : 80 ml Wash Buffer (concentrated): 25 ml Elution Buffer : 6 ml (10mM Tris-HCI,pH8.5 at 25℃) Wash Buffer(concentrated) : 25 ml Elution Buffer :6 ml RNase A (50mg/ml):50μg PD columns : 100pcs Collection Tubes : 100 pcs	PD1 Buffer : 65 ml PD2 Buffer : 75 ml PD3 Buffer : 100 ml W1 Buffer : 130 ml Wash Buffer(concentrated) : 50 ml Elution Buffer :30 ml RNase A (50mg/ml):130μg PD columns : 300pcs Collection Tubes : 300 pcs