Introduction
Format: Spin columns
Sample: Plant tissues
Sample size: Up to 100mg / 1g fresh sample
or 20mg / 200mg dry sample
Operation: Centrifuge / vacuum manifold
Operation time: 30~60 minutes
Binding capacity: 50µg~500µg DNA
Application: PCR、Real-time PCR、Southern blotting、AFLP、PADP/ AFLP
 


ATP™Plant Genomic DNA Mini/Maxi Kit provides a fast and simple method to isolate total DNA (genomic DNA, mitochondrial, chloroplast and viral DNA) from plant tissue and cells.

1.  Plant tissues are ground in liquid nitrogen and lysed by a buffer containing detergent .

2.  The tissue debris in lysate could be removed by the provided filter column.

3.  In the presence of a chaotropic salt, the genomic DNA in the lysate binds to the glass fibre matrix in the spin column.

4.  After washing off the contaminants, the purified DNA is eluted by a low salt elution buffer or water.

Sample

DNA yield (μg)

Mini(100mg leaf)

Maxi( 1g leaf)

Arabidopsis ( Arabidopsis thaliana )

3~5

30~50

Bamboo ( Bambusa lodhamii )

10~15

100~150

Camphor tree( Cinnamommun camohora )

15~20

150~200

Chianes Yam ( Rhizoma dioscoreae )

30~60

300~500

Maize ( Zea mays )

15~20

150~200

Orchis ( Phalaenopsis aphrodite )

5~10

50~100

Rice( Oryza sativa )

10~15

100~150

Spinach ( Spinacia oleracea )

5~10

50~100

Sweet potato ( Ipomoea batata )

20~30

200~300

Tobaco ( Nicotiana tabacum )

20~25

200~250

Tomato ( Lycopersicon esculentum )

10~15

100~150



 
Quality Control

The quality of ATP™ Genomic DNA Kit (Plant) is tested on a lot-to-lot basis. The Kits are tested by isolation of genomic DNA from 50mg young leave. The purified DNA is quantified with spectrophotometer and the yield of genomic DNA is more than 10 μg with A260/A280 ratio 1.7 to 1.9.

 
Kit Contents:Cat.No. / Kit Contents
XXX
XXX
123
123
 
 
 
 
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
 
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
 
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
 
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
 
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
 
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis