ATP™Plant Genomic DNA Mini/Maxi Kit provides a fast and simple method to isolate total DNA (genomic DNA, mitochondrial, chloroplast and viral DNA) from plant tissue and cells.
1. Plant tissues are ground in liquid nitrogen and lysed by a buffer containing detergent .
2. The tissue debris in lysate could be removed by the provided filter column.
3. In the presence of a chaotropic salt, the genomic DNA in the lysate binds to the glass fibre matrix in the spin column.
4. After washing off the contaminants, the purified DNA is eluted by a low salt elution buffer or water.
Sample |
DNA yield (μg) |
Mini(100mg leaf) |
Maxi( 1g leaf) |
Arabidopsis ( Arabidopsis thaliana ) |
3~5 |
30~50 |
Bamboo ( Bambusa lodhamii ) |
10~15 |
100~150 |
Camphor tree( Cinnamommun camohora ) |
15~20 |
150~200 |
Chianes Yam ( Rhizoma dioscoreae ) |
30~60 |
300~500 |
Maize ( Zea mays ) |
15~20 |
150~200 |
Orchis ( Phalaenopsis aphrodite ) |
5~10 |
50~100 |
Rice( Oryza sativa ) |
10~15 |
100~150 |
Spinach ( Spinacia oleracea ) |
5~10 |
50~100 |
Sweet potato ( Ipomoea batata ) |
20~30 |
200~300 |
Tobaco ( Nicotiana tabacum ) |
20~25 |
200~250 |
Tomato ( Lycopersicon esculentum ) |
10~15 |
100~150 |
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