Format: Spin columns
Sample: 200μl of serum, plasma, body fluids and cell culture supernatant
Operation: Centrifuge / vacuum manifold
Operation time: 40 minutes
Elution volume: 50μl
Application: RT-PCR, PCR, Real-time PCR, Real-time RT-PCR, Automated fluorescent DNA sequencing.

ATP™ Viral Nucleic Acid Extraction Kit is specially designed for high-throughput purification of viral RNA or DNA from cell-free samples as serum, plasma, body, fluids, and the supernatant of viral infected cell culture. With the extraction method, DNA/RNA viruses are lysed quickly and efficiently by lysis buffer which is a highly concentrated solution of chaotropic salt. Nucleic acid in chaotropic salt and methanol are bond to the glass-fiber matrix of viral DNA/RNA Binding Column. Contamina-tions like salts, metabolites and soluble macromolecular components are removed in Washing Steps. The nucleic acids can be eluted by low-salt buffer or water and are ready-to-use in subsequent reactions. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assay. This protocol is recommended for parallel purification of viral RNA including HCV , HIV and viral DNA including HBV and CMV.

Kit Contents:Cat.No. / Kit Contents
AVR050 (50 preps/kit)
AVR100 (100 preps/kit)
VB Lysis Buffer: 30 ml
AD Buffer(concentrated) : 4 ml
W1 Buffer : 30 ml
Wash Buffer(concentrated) : 12.5 ml
RNase-free water:6 ml
VB columns : 50 pcs (yellow/white filter)
2ml Collection Tubes : 100 pcs
VB Lysis Buffer: 60 ml
AD Buffer(concentrated) : 8 ml
W1 Buffer : 50 ml
Wash Buffer(concentrated) : 25 ml
RNase-free water:6 ml
VB columns : 100 pcs (yellow/white filter)
2ml Collection Tubes : 200 pcs
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis