ATP™ Viral Nucleic Acid Extraction Kit is specially designed for high-throughput purification of viral RNA or DNA from cell-free samples as serum, plasma, body, fluids, and the supernatant of viral infected cell culture. With the extraction method, DNA/RNA viruses are lysed quickly and efficiently by lysis buffer which is a highly concentrated solution of chaotropic salt. Nucleic acid in chaotropic salt and methanol are bond to the glass-fiber matrix of viral DNA/RNA Binding Column. Contamina-tions like salts, metabolites and soluble macromolecular components are removed in Washing Steps. The nucleic acids can be eluted by low-salt buffer or water and are ready-to-use in subsequent reactions. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assay. This protocol is recommended for parallel purification of viral RNA including HCV , HIV and viral DNA including HBV and CMV.