Introduction

Format: Maxi Spin column
Sample: 100-200 mg animal tissue
107-108 cultured cell 5 ml blood sample
Operation: Centrifuge
Operation time: < 60 minutes
Elution volumw: 500 μl
Yield : 500 μg
Application: RT-PCR、Real-time PCR、Notthern blotting、mRNA selection、cDNA synthesis、Primer extension

 


ATP™ Total RNA Maxi Kit provides a fast and simple method to isolate total RNA from animal tissue and cells. In the process, sample is distrusted by grinding in liquid nitrogen and filtrated by filter column to remove cell debris. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column. The optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer. Finally, the purified total RNA is eluted by RNase-free water. The protocol does not require phenol extraction and alcohol precipitation. The entire procedure can be completed in 60 minutes.


 
Quality Control
 
Kit Contents:Cat.No. / Kit Contents
ARBM10 (10preps/ kit )
RBC lysis Buffer :200 ml
RT Buffer :30 ml
RB Buffer :60 ml
W1 Buffer :50 ml
Wash Buffer (concentrated)*:25 ml
RNase-free water:6 ml
Filter Column : 10 pcs
RB-Maxi Columns:10pcs
 
 
 
 
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
 
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
 
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
 
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
 
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
 
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis