ATP biotech Inc.

Introduction

Format: Maxi Spin column
Sample: 100-200 mg animal tissue
107-108 cultured cell 5 ml blood sample
Operation: Centrifuge
Operation time: ＜ 60 minutes
Elution volumw: 500 μl
Yield : 500 μg
Application: RT-PCR、Real-time PCR、Notthern blotting、mRNA selection、cDNA synthesis、Primer extension

ATP™ Total RNA Maxi Kit provides a fast and simple method to isolate total RNA from animal tissue and cells. In the process, sample is distrusted by grinding in liquid nitrogen and filtrated by filter column to remove cell debris. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column. The optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer. Finally, the purified total RNA is eluted by RNase-free water. The protocol does not require phenol extraction and alcohol precipitation. The entire procedure can be completed in 60 minutes.

Quality Control

Kit Contents：Cat.No. / Kit Contents

ARBM10 (10preps/ kit )
RBC lysis Buffer ：200 ml RT Buffer ：30 ml RB Buffer ：60 ml W1 Buffer ：50 ml Wash Buffer (concentrated)*：25 ml RNase-free water：6 ml Filter Column ： 10 pcs RB-Maxi Columns：10pcs