Introduction
Format: Spin columns
Sample: Animal tissues, mouse tails, swabs, blood and other body fluids
Sample size: Up to 50mg tissue or 200µl blood
Operation: Centrifuge / vacuum manifold
Operation time: 30~60 minutes
Binding capacity: 50µg DNA
Application: PCR、Real-time PCR、Southern blotting、AFLP、PADP/ AFLP

 

ATP™ Genomic DNA Mini Kit is specially designed for purification of total DNA (including : Genomic, mitochondrial and viral DNA ) from a variety of animal tissues or cells.

1.  Provided micropestle can efficiently homogenize tissue samples to shorten the time on the lysis step.

2.  The method uses proteinase K and chaotropic salt to lyse cells and degrade protein, and then DNA in chaotropic salt is bound to the glass fiber matrix of the column.

3.  After washing off the contaminants, the purified DNA is eluted by a low salt elution buffer or water.

Expectant yield:




 
Quality Control

The quality of ATP™ Genomic DNA Kit (Tissue) is tested on a lot-to-lot basis. The Kits are tested by isolation of genomic DNA from 10mg of mouse liver. The purified DNA is quantified with spectrophotometer and the yield of genomic DNA is more than 10 μg with A260/A280 ratio 1.7 to 1.9.

 
Kit Contents:Cat.No. / Kit Contents
AGT50(50 prep/kit)
APD300 (300 preps/kit)
GT Buffer:30 ml
GB Buffer:30 ml
W1 Buffer:45 ml
Wash Buffer(concentrated):25 ml
Elution Buffer:30 ml
Proteinase K:11 mg
GD Columns:50 pcs
Collection Tubes:100 pcs
Micropestle:50 pcs
GT Buffer:75 ml
GB Buffer:75 ml
W1 Buffer:130 ml
Wash Buffer(concentrated):50 ml
Elution Buffer:75 ml
Proteinase K:66 mg
GD Columns:300 pcs
Collection Tubes:600 pcs
Micropestle:300 pcs
 
 
 
 
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
 
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
 
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
 
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
 
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
 
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis