ATP biotech Inc.

Introduction

Format: 96-Well
Sample: 00mg agarose gel slice；10~50µl PCR product or other enzymatic reaction
Operation: Centrifuge / vacuum manifold
Operation time: 30 minutes
Elution volume: 50μl
Application: DNA Sequencing；Ligation；PCR；Restriction Digestion；DNA labeling
For research use only ； Not for diagnostic or drug purposes

ATP™96-Well PCR Clean Up Kit provides a high-throughput, rapid and economical method to purify DNA fragments.

1. The method utilizes a chaotrophic salt to denature enzymes and in this condition DNA fragment is absorbed to the fibre matrix in each well of the plate.

2. Following washing of contaminants, the purified DNA is eluted by low salt buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reactions mixtures without toxic phenol extraction and alcohol precipitation.

The entire procedure can be completed in 30 minutes.

Quality Control

The quality of 96-Well Gel/PCR DNA Extraction Kit is tested on a lot-to-lot basis. The efficiency of DNA recovery is tested by isolation of DNA fragments of various sizes. The purified DNA is checked by agarose gel analysis.

Kit Contents：Cat.No. / Kit Contents

ADF9602 (2 x 96prep / kit )	ADF9604 (4 x 96prep / kit )	ADF9610 (10 x 96prep / kit )
Binding Buffer ：80ml Wash Buffer (concentrated)：25ml X 1 Elution Buffer：30ml 96-Well binding plate：2pcs 0.35 ml Collection Tubes：2pcs Adhesive film：4pcs	Binding Buffer ：120ml Wash Buffer (concentrated)：25ml X 2 Elution Buffer：30ml 96-Well binding plate：4pcs 0.35 ml Collection Tubes：4pcs Adhesive film：8pcs	Binding Buffer ：240ml + 80ml Wash Buffer (concentrated)：50ml X 2 Elution Buffer：60ml 96-Well binding plate：10pcs 0.35 ml Collection Tubes：10pcs Adhesive film：20pcs