ATP™96-Well PCR Clean Up Kit provides a high-throughput, rapid and economical method to purify DNA fragments.
1. The method utilizes a chaotrophic salt to denature enzymes and in this condition DNA fragment is absorbed to the fibre matrix in each well of the plate.
2. Following washing of contaminants, the purified DNA is eluted by low salt buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reactions mixtures without toxic phenol extraction and alcohol precipitation.
The entire procedure can be completed in 30 minutes.