ATP biotech Inc.

Introduction

Format: 96-well plate
Sample: 1~2 ml bacterial cultures
Operation: centrifuge / vacuum manifold
Operation time: 60 minutes
Binding capacity: 10μg per well
Expected yield: 0.5~5μg for high-copy plasmid
5~10μg per low-copy plasmid
Application: DNA Library Screening and Analysis
DNA Sequencing
Transformation
Restriction Digestion

ATP™96-well Plasmid Kit is designed for high-throughput plasmid or cosmid DNA from 1~2ml of bacterial cultures.

1. There are all the necessary plates, buffers and reagents for ready-use in the kit.

2. Binding, washing and elution steps can be done using a vacuum manifold or by centrifugation.

3. In the process, the modified alkaline lysis method and centrifugation are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants.

Quality Control

The quality of ATP™ Plasmid Mini Kit is tested on a lot-to-lot basis. The Kits are tested by isolation of plasmid DNA from 4 ml culture of E.coli DH5α transformed with the plasmid pBluescript (A600 >2 units/ml). More than 25 μg of plasmid DNA could be quantified with spectrophotometer. 1 µg of the purified plasmid is used on restriction enzyme digestion with Eco RI and digested DNA is checked by agarose gel analysis.

Kit Contents：Cat.No. / Kit Contents

APD100 (100 preps/kit)	APD300 (300 preps/kit)
PD1 Buffer : 25 ml PD2 Buffer : 25 ml PD3 Buffer : 45 ml W1 Buffer : 45 ml Wash Buffer(concentrated) : 25 ml Elution Buffer :6 ml RNase A (50mg/ml):50μg PD columns : 100pcs Collection Tubes : 100 pcs	PD1 Buffer : 65 ml PD2 Buffer : 75 ml PD3 Buffer : 100 ml W1 Buffer : 130 ml Wash Buffer(concentrated) : 50 ml Elution Buffer :30 ml RNase A (50mg/ml):130μg PD columns : 300pcs Collection Tubes : 300 pcs