Format: Maxi Spin column
Sample: Up to 4 ml of fresh blood for AGBM25
Up to 10 ml of frozen blood for AGDM25
Operation: Centrifuge / vacuum manifold
Application: PCR、Real-time PCR、Southern blotting、AFLP、PADP/ AFLP

ATP™Genomic DNA Maxi Kit (Blood / Cultured Cell) provide a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood, plasma, serum, buffy coat, other body fluids, lymphocytes, bacterial and cultured cells. In AGBM25, RBC Lysis Buffer is used to remove non-nucleated red blood cells and reduce hemoglobin contamination. In AGDM25, Proteinase K is the optimal enzyme to cell lysis for frozen blood. The method use a chaotropic salt, guanidine hydrochloride to lyse cells and degrade protein, than DNA in chaotropic salt is bond to glass fiber matrix of column(1). After washing off the contaminants, the purified genomic DNA is eluted by low salt elution buffer or water. The entire procedure can be completed without phenol/chloroform extraction and alcohol precipitation. Average yields of AGBM25 / AGDM25 are up to 140 µg of DNA from 4/10 ml of fresh/frozen blood. Purified DNA with approximate 20-30kb is suitable for PCR or other enzyme reaction.

Quality Control

The quality of ATP™ Genomic Maxi DNA Kit (Blood/Culture Cell) is tested on a lot-to-lot basis. The Kits are tested by isolation of genomic DNA from 4 ml of human whole blood. The purified DNA is quantified with spectrophotometer and the yield of genomic DNA is > 100 μg with A260/A280 ratio 1.6 to 1.8.

Kit Contents:Cat.No. / Kit Contents
AGBM10 (25preps/ kit )
AGDM10 (25preps/ kit )
RBC lysis Buffer :360ml
GB Buffer :60ml
W1 Buffer :60ml
Wash Buffer (concentrated)*:25ml
Elution Buffer:30ml
GD Maxi Columns:25pcs
Proteinase K :130mg
GB Buffer :275ml
W1 Buffer :130ml
Wash Buffer (concentrated)*:50ml
Elution Buffer:60ml
GD Maxi Columns:25pcs
Plasmid Extraction
Plasmid Mini Kit
Plasmid Midi Kit (Ultra Pure)
Plasmid Maxi Kit (Ultra Pure)
96-Well Plasmid Mini Kit
Plasmid Binding Column
Plasmid Midi/Maxi Column
Fragment DNA Extraction
Gel/PCR DNA Extraction Kit
96-Well Gel/PCR DNA Extraction Kit
Fragment DNA Binding Column
Genomic DNA Extraction
Reagent Genomic DNA Kit
Genomic DNA Mini
(Blood/Culture Cell/Bacteria) Kit
Genomic DNA Mini (Tissue) Kit
Genomic DNA Mini (Plant) Kit
Genomic DNA Maxi (Fresh Blood) Kit
Genomic DNA Maxi (Frozen Blood) Kit
Genomic DNA Maxi (Plant) Kit
96-Well Genomic DNA Kit
Genomic DNA Binding Column
RNA Extraction
Total RNA Mini
(Blood/Culture Cell/Bacteria) Kit
RNA Mini (Tissue) Kit
Total RNA Mini (Plant) Kit
RNA Maxi
(Blood/Culture Cell/Tissue) Kit
RNA Maxi (Plant) Kit
RNA Binding Column
Viral Nucleic Acid Extraction
Viral Nucleic Acid Mini Kit
96-Well Viral Nucleic Acid Mini Kit
Cloning Service
Custom Gene Cloning
Plasmid DNA Preparation
RNA/mRNA Isolation Service
Site-directed Mutagenesis