ATP biotech Inc.

Introduction

Format: Maxi Spin column
Sample: Up to 4 ml of fresh blood for AGBM25
Up to 10 ml of frozen blood for AGDM25
Operation: Centrifuge / vacuum manifold
Application: PCR、Real-time PCR、Southern blotting、AFLP、PADP/ AFLP

ATP™Genomic DNA Maxi Kit (Blood / Cultured Cell) provide a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood, plasma, serum, buffy coat, other body fluids, lymphocytes, bacterial and cultured cells. In AGBM25, RBC Lysis Buffer is used to remove non-nucleated red blood cells and reduce hemoglobin contamination. In AGDM25, Proteinase K is the optimal enzyme to cell lysis for frozen blood. The method use a chaotropic salt, guanidine hydrochloride to lyse cells and degrade protein, than DNA in chaotropic salt is bond to glass fiber matrix of column(1). After washing off the contaminants, the purified genomic DNA is eluted by low salt elution buffer or water. The entire procedure can be completed without phenol/chloroform extraction and alcohol precipitation. Average yields of AGBM25 / AGDM25 are up to 140 µg of DNA from 4/10 ml of fresh/frozen blood. Purified DNA with approximate 20-30kb is suitable for PCR or other enzyme reaction.

Quality Control

The quality of ATP™ Genomic Maxi DNA Kit (Blood/Culture Cell) is tested on a lot-to-lot basis. The Kits are tested by isolation of genomic DNA from 4 ml of human whole blood. The purified DNA is quantified with spectrophotometer and the yield of genomic DNA is > 100 μg with A260/A280 ratio 1.6 to 1.8.

Kit Contents：Cat.No. / Kit Contents

AGBM10 (25preps/ kit )	AGDM10 (25preps/ kit )
RBC lysis Buffer ：360ml GB Buffer ：60ml W1 Buffer ：60ml Wash Buffer (concentrated)*：25ml Elution Buffer：30ml GD Maxi Columns：25pcs	Proteinase K ：130mg GB Buffer ：275ml W1 Buffer ：130ml Wash Buffer (concentrated)*：50ml Elution Buffer：60ml GD Maxi Columns：25pcs