ATP™ Plant Total RNA Mini Kit provides a fast and simple method to isolate total RNA from plant tissue and cells.
1. In the process, sample is first ground in liquid nitrogen and filtrated by filter column to remove cell debris.
2. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column.
3. The optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer.
4. Finally, the purified total RNA is eluted by RNase-free water.
The protocol does not require phenol extraction and alcohol precipitation.