ATP biotech Inc.

Introduction

Format: Spin columns
Sample: 50 mg plant tissue
Operation: Centrifuge / vacuum manifold
Operation time: ＜ 60 minutes
Yield: 5-30 μg
Elution volume: 50 µl
Application: RT-PCR、Real-time PCR、Notthern blotting、mRNA selection、cDNA synthesis、Primer extension

ATP™ Plant Total RNA Mini Kit provides a fast and simple method to isolate total RNA from plant tissue and cells.

1. In the process, sample is first ground in liquid nitrogen and filtrated by filter column to remove cell debris.

2. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to glass fiber matrix in the spin column.

3. The optional DNase treatments can remove DNA residues and the contaminants are washed with an ethanol contained wash buffer.

4. Finally, the purified total RNA is eluted by RNase-free water.

The protocol does not require phenol extraction and alcohol precipitation.

Quality Control

The quality of Plant Total RNA Mini Kit is tested on a lot-to-lot basis. The Kits are tested by isolation of total RNA from 25 mg young leaf. Purified RNA could be quantified with spectrophotometer and checked by agarose gel.

Kit Contents：Cat.No. / Kit Contents

ARP050 (50prep/kit )	ARP100 (100 prep/kit )
RB Buffer ：30ml PRB Buffer ：30ml W1 Buffer ：30ml Wash Buffer (concentrated)*：12.5ml RNase-free water：6 ml RB Columns：50 pcs (yellow/white filter) Filter Column：50 pcs 2 ml Collection Tubes：100pcs	RB Buffer ：60ml PRB Buffer ：30ml W1 Buffer ：50ml Wash Buffer (concentrated)：25ml RNase-free water：6 ml RB Columns：100 pcs (yellow/white filter) Filter Column：100 pcs 2 ml Collection Tubes：200pcs